DETECTION OF FASCIOLA HEPATICA USING NESTED-PCRIN THE SLAUGHTERHOUSES OF ALBORZ, IRANA. REZAEE KALVANI1, L. ABDOLMOHAMMADI KHIAV1 & S. R. HOSSEINI21Razi Vaccine and Serum Research Institute, Agricultural Research, Education and ExtensionOrganization (AREEO), Tehran, Iran; 2Department of Pathobiology, Faculty of VeterinaryMedicine Islamic Azad University, Shahrekord Branch, Shahrekord, IranSummaryRezaee Kalvani, A., L. Abdolmohammadi Khiav & S. R. Hosseini, 2023. Detection of Fasciolahepatica using nested-PCR in the slaughterhouses of Alborz, Iran. Bulg. J. Vet. Med.(online first).Fasciola hepatica is a parasitic liver trematode that causes fasciolosis in humans and dairy animals.Traditional detection of infection is based on a microscopic examination with low sensitivity. Therefore,this study was carried out to develop an accurate and rapid method to detect F. hepatica in Alborzprovince. In this cross-sectional study, 386 samples were collected from livers of cattle andsheep in three slaughterhouses of Alborz. DNA was extracted, and nested-PCR was carried out basedon the F. hepatica ITS-2 sequence. DNA amplification showed first and second PCR products withexpected size of 336 and 208 bp respectively, as unique for F. hepatica. The results showed no crossreactionwith the negative control. Thirty-five liver samples were positive for this parasitic infection.Totally, the prevalence of F. hepatica in the slaughterhouses of Alborz, Iran was 9.07%. This is thefirst study o kalvan كلوان........ (كلوان)............
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برچسب : نویسنده : alikalvana بازدید : 10 تاريخ : دوشنبه 13 شهريور 1402 ساعت: 9:37